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Bioluminescence spectra in COS-1 cells transfected with Macrolampis firefly luciferase carrying vector, pCMV-Mac, and ratiometric curves. Effect of pH in cells transfected with: (A) pCMV-Mac;cytoplasm; (B) pCMV-Mac-Nucleus; (C) pCMV-Mac with calibration buffer containing nigericin at different pHs (pH 6.0, 6.5, 7.0, 7.5 and 8.0); (D) ratiometric analysis between R (Igreen/Ired) and pH. Reprinted with permission from ref. [34]. Copyright 2019 Royal Society of Chemistry.
