Figure 9.

Comparison of the effect of temperature on the ratio of bioluminescence color of beetle luciferases: (left graph) ratiometric estimation of temperature using distinct firefly luciferases. (red) Macrolampis sp2; (black) Photinus pyralis; (green) Cratomorphus distinctus, and (blue) Amydetes viviani; (right images) in vivo bioluminescence color of E.coli colonies expressing Macrolampis sp2 (Mac-Luc) and Amydetes viviani (Amy-Luc) luciferases, after spraying D-luciferin, at 22 and 40 °C. Reprinted with permission from ref. [85]. Copyright 2014 Royal Society of Chemistry. One can see that the luciferases with steeper curves such as Macrolampis luciferase (red) are the most sensitive to temperature, and those with less steep curves, such as Amydetes viviani luciferase (blue), are the least sensitive.