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. 2022 Jun 10;12(6):400. doi: 10.3390/bios12060400

Table 1.

pH-sensing and metal binding properties of firefly luciferasesand their engineered forms, and potential applicability.

Luciferase RA (%) pH Sensitivity (nm) RA ZnSO4 1 mM (%) ZnSO4 Detection Limit * (µM) ZnSO4 Spectral Shift (nm) ** RA CdSO4 1 mM (%) CdSO4 Detection Limit * (µM) CdSO4 Spectral Shift (nm) ** RA HgCl2 1 mM (%) HgCl2 Detection Limit * (µM) HgCl2 Spectral Shift (nm) ** Applicability
Macrolampis sp2 100 569–616 77 500 9 36 890 19 18 170 34 pH-indicator
N354H 5.7 568–615 20 110 30 21 2000 5 7 150 37 pH-indicator
N354C 75 564–606 8 20 47 13 15 50 6 15 37 Zinc, Cadmium and Mercury enzymatic sensor
H310C 62 573–613 20 100 15 - 3500 0 11 90 34 pH-indicator
H310C/N354 - - 15 15 34 11 260 41 4 30 36 Zinc and Mercury enzymatic sensors
N354E - - 90 225 23 14 4000 0 7 130 21
Cratomorphus distinctus 100 554–614 - - 36 - - - - - 46 pH-indicator
Amydetes viviani 100 549–596 2 2000 4 4 100 29 1,5 60 33 Cadmium and Mercury enzymatic sensors; thermostable pH indicator

* The detection limit is the concentration of a given metal which red-shifts the spectrum by no less than 5 nm. ** Maximum spectral shift observed at the metal concentration of 2 mM.