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Substrate specificity of thermostable chitosanase from Bacillus sp. strain CK4
| Substrate (1.0%) | Total activity (U) | Relative activitya (%) | Vmax (U/mg) | Km (mg/ml) |
|---|---|---|---|---|
| Chitinb | 0 | 0 | 0 | |
| Colloidal chitin | 0 | 0 | 0 | |
| Glycol chitin | 0 | 0 | 0 | |
| Soluble chitosan | 56.8 | 100 | 173 | 0.8 |
| Colloidal chitosan | 23.3 | 41 | 71.5 | 8.7 |
| Glycol chitosan | 9.7 | 17 | 27.9 | 23.9 |
Expressed as a percentage of the activity measured with soluble chitosan.
The purified chitin was purchased from Sigma.
