TABLE 2.
Substrate specificity of thermostable chitosanase on chitosan with various DDA
| Substrate (1.0%) and DDA (%)a | Total activity (U) | Relative activityb (%) | Vmax (U/mg) | Km (mg/ml) |
|---|---|---|---|---|
| Soluble chitosan | ||||
| 99 | 56.8 | 100 | 173 | 0.8 |
| 83 | 52.3 | 92.0 | 151 | 1.2 |
| 71 | 36.4 | 64.0 | 155 | 3.4 |
| 53 | 25.0 | 44.1 | 99.7 | 6.8 |
| Colloidal chitosan | ||||
| 99 | 13.1 | 23.0 | 71.5 | 8.7 |
| 83 | 21.1 | 37.1 | 152 | 5.8 |
| 71 | 25.0 | 44.0 | 132 | 3.7 |
| 53 | 40.2 | 70.7 | 160 | 0.5 |
a
DDA were calculated by using the colloidal titration method (36).
b
Expressed as a percentage of the activity measured with soluble chitosan with a DDA of 99%.