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. 2022 Jun 17;14(12):2986. doi: 10.3390/cancers14122986

Figure 5.

Figure 5

Non-tumoral and tumoral parasympathetic paraganglia express high levels of EPAS1/HIF2α. (a,b) The transcriptional levels of the indicated HIF1α (a) and HIF2α (b) target genes were analyzed in HNPGL lacking (others) or carrying mutations in SDH or VHL genes using the Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays [24]. (c) Representative images of HIF2α and HIF1α immunohistochemistry performed in non-tumoral CB and in HNPGL showing nuclear (HIF2αNUC) or cytoplasmic (HIF2αCYT) immunostaining. Immunostaining of S100, marker of sustentacular cells, and tyrosine hydroxylase (TH), marker of neuron-like glomus type I cells, are also shown. Insets show magnified images to better visualize glomus type I cells and sustentacular cells. Scale bars, 100 μm. (d) Distribution of the mRNA abundance of the whole genome gene expression data of non-tumoral CB obtained from the Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays [21]. The level of EPAS1 mRNA is indicated by an arrow. (e) Transcriptional levels of EPAS1 and HIF1A in HNPGL lacking (others) or carrying mutations in SDH or VHL and in normal CB (NT). * p < 0.01, ** p < 0.005, **** p < 0.0001.