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. 2022 Jun 20;12(6):854. doi: 10.3390/biom12060854

Figure 3.

Figure 3

CBD and CBG treatment promoted apoptosis and autophagy in HuCC-T1 cells; (A,B). annexin V (FITC-H) and propidium iodide (PI) co-staining flow cytometry analysis showing an increase in late apoptotic cells and a significant decrease in live cells upon CBG treatment; (C). DAPI staining of nucleus (blue) and Alex Fluor 555 staining of BAX (red) for 0.1% DMSO (top), 100 μM CBD (middle) and 100 μM CBG (bottom) at 24 h post treatment; (D). graph showing quantification of BAX fluorescence in all three groups with a significant change in BAX fluorescence with CBG treatment. CTCF: corrected total cell fluorescence. * p < 0.05, ** p < 0.01, *** p < 0.001.