Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 2;12(6):777. doi: 10.3390/biom12060777

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

ChT-L peptidase of proteasome was activated up to 3-fold with 50 µM M-5 and M-7 in cell extracts prepared from HEK293T cells. M-12 failed to stimulate the ChT-L peptidase in cell lysates, exactly as with the purified 20S proteasome. The selective proteasome inhibitor, 1 µM bortezomib, used as a negative control, completely blocked the cell capacity of degrading Suc-LLVY-AMC substrate. The results are presented as the mean of three independent replicates ± SEM. Ordinary one-way ANOVA analysis was used to determine statistical significance (* p < 0.05).