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View full-text article in PMC

. 2022 Jun 10;14(12):2871. doi: 10.3390/cancers14122871

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determining CTC by ICC×2 in endometrial and ovarian cancers: CTCs were captured from blood samples from patients with endometrial (A) and ovarian (B) tumors and enumerated using ICC×2 (Ai,Bi). Blood samples were spiked (Spiked samples) with titrating number (250 cells/100 cells) of NCI-H441 cells separately for ICC×2. Corresponding CTC enumeration by IF×3 (Aii,Bii) is presented. For IF×3, pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. For ICC×2, pictures were taken at 40× objective of an Olympus BX43 Microscope. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus. The immunocytochemistry-photomicrographs are presented with a scale bar, magnification information, digital reticule, as well as the diameters (μm) of CTC and a representative WBC.

Determining CTC by ICC×2 in endometrial and ovarian cancers: CTCs were captured from blood samples from patients with endometrial (A) and ovarian (B) tumors and enumerated using ICC×2 (Ai,Bi). Blood samples were spiked (Spiked samples) with titrating number (250 cells/100 cells) of NCI-H441 cells separately for ICC×2. Corresponding CTC enumeration by IF×3 (Aii,Bii) is presented. For IF×3, pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. For ICC×2, pictures were taken at 40× objective of an Olympus BX43 Microscope. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus. The immunocytochemistry-photomicrographs are presented with a scale bar, magnification information, digital reticule, as well as the diameters (μm) of CTC and a representative WBC.