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. 2022 Jun 7;11(12):1865. doi: 10.3390/cells11121865

Figure 1.

Figure 1

Conditional deletion of Myh9 leads to pancytopenia and fatal bone marrow failure. (A) Generation of Myh9 conditional knockout mice. The exon1 of Myh9 is targeted by inserting two LoxP sites to create Myh9 floxed allele. The Myh9 floxed allele can be deleted by the expression of Cre recombinase. (B) Kaplan-Meier analysis showed marked decrease in survival of Myh9fl/fl:Mx1-cre mice compared with control mice after first poly I:C injection (n = 20 each group). (C) Real time PCR analysis for Myh9 mRNA showed efficient deletion of Myh9 in the BM of Myh9fl/fl:Mx1-cre after induction with poly I:C (n = 3). (D) Complete blood count analysis (CBC) of peripheral blood from Myh9fl/fl:Mx1-cre and control mice 10 days after injection of poly I:C, white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelet (PLT), lymphocyte (LYMPHO), monocyte (MONO), neutrophil (NE) (n = 5). (E) BM cells were significantly reduced in Myh9fl/fl:Mx1-cre mice compare to control mice 10 days after poly I:C induction (n = 5). All data are shown as mean ± SD. Student t test was used to compare two groups of mice (** p < 0.005, *** p < 0.001, **** p < 0.0001). (F) Peripheral blood smear (500×) from control and Myh9fl/fl:Mx1-cre mice at 10 days after poly I:C injection. The Myh9fl/fl:Mx1-cre blood smear showed severe anemia. Bar value = 20 μm.