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. 2022 Jun 10;11(12):1893. doi: 10.3390/cells11121893

Figure 6.

Figure 6

Depletion of Ly6G+ cells protects against Type IIx/b muscle fiber atrophy within diaphragm muscles of KPC mice. (A) Representative images of diaphragm strips stained with WGA to label extracellular matrix (yellow), and antibodies against myosin heavy chain I (green) and MHC IIa (blue). Type IIb and IIx fibers were identified as unstained (black) fibers. (B) Quantification of average, and fiber type-specific muscle fiber size, based on measurement of the minimum Feret’s diameter (MFD). A minimum of 1000 randomly selected fibers were analyzed per mouse. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = not significant (p > 0.05). Control: n = 8; IgG2a n = 5; Ly6G Ab: n = 4. Scale bar = 100 μm. (C) Fiber-type proportions in diaphragm muscles from Sham control mice, and KPC tumor-bearing mice. A minimum of 1000 randomly selected fibers were analyzed per mouse. * p < 0.05; ** p < 0.01; ns = not significant (p > 0.05).