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Tc(VII) reduction and precipitation via microbially produced Fe(II) and AQHDS. Washed cell suspensions of G. sulfurreducens were incubated with 250 μM Tc(VII), 20 mM acetate (electron donor), and either 25 mM Fe(III) citrate, 100 mM Fe(III) oxide, 50 μM mM AQDS, or 50 μM mM AQDS and 100 mM Fe(III) oxide. The control had no Fe(III) or AQDS. Reduced insoluble Tc was removed from solution by centrifugation prior to analysis using a phosphorimager (19).
