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. 2022 May 29;12(6):759. doi: 10.3390/biom12060759

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) The RP-HPLC chromatogram of the skin secretion of Pelophylax kl. esculentus. The peak of temporin-PKE is indicated by an arrow. (b) The annotated LCQ tandem mass (MS/MS) fragmentation spectrum of temporin-PKE. Prominent ions in are labeled with the observed m/z value and ion series designation. b and y denote the N- and C-terminal fragments of the peptide produced by breakage at the peptide bond in LCQ, respectively. The number represents the residue number from either the N- or C- terminus. (c) Electrospray ion-trap MS/MS fragmentation data derived from fragment ions in panel (b) corresponding in molecular mass to temporin-PKE. Predicted singly- and doubly-charged ions are coloured black. The actual b-ions and y-ions detected by MS/MS fragmentation are indicated in red and blue typeface, respectively.

(a) The RP-HPLC chromatogram of the skin secretion of Pelophylax kl. esculentus. The peak of temporin-PKE is indicated by an arrow. (b) The annotated LCQ tandem mass (MS/MS) fragmentation spectrum of temporin-PKE. Prominent ions in are labeled with the observed m/z value and ion series designation. b and y denote the N- and C-terminal fragments of the peptide produced by breakage at the peptide bond in LCQ, respectively. The number represents the residue number from either the N- or C- terminus. (c) Electrospray ion-trap MS/MS fragmentation data derived from fragment ions in panel (b) corresponding in molecular mass to temporin-PKE. Predicted singly- and doubly-charged ions are coloured black. The actual b-ions and y-ions detected by MS/MS fragmentation are indicated in red and blue typeface, respectively.