Figure 9.

The membrane permeability of temporin-PKE and its analogues against (a) S. aureus and (b) E. coli. N represents the negative control, which is 5% TSB in 0.85% NaCl and 5% LB in 0.85% NaCl, respectively. P represents the positive control, which is 8µM mellitin and 70% Isopropanol, respectively. The results were analysed by two-way ANOVA and Dunnett’s multiple comparisons test compared the cell viability of different concentrations of peptides with that of the negative control group. The significance is indicated by **** which means p < 0.0001. The error bar represents the SEM of the nine replicates from three independent tests.