Figure 4.

Camkk2 knockout decreases high-fat diet-induced metastatic colonization of NEPC in TRAMP mice. (A) Schema for mouse experiment in which C57BL/6 TRAMP;Camkk2^+/+ and TRAMP;Camkk2^−/− mice were fed either a high-fat diet (HFD) or normal diet (ND) (HFD WT n = 8; HFD KO n = 8; ND WT n = 9; ND KO n = 10). (B) % low-grade PIN (LGPIN), high-grade PIN (HGPIN), and cancer incidence among cohorts. Lung metastasis was quantified by counting incidents of (C) micrometastases (area of metastasis is <10.000 µm²), (D) large metastases (area of metastasis is > 10.000 µm²), or (E) any metastatic lesions. In addition, (F) image analysis was performed on lung H&E slides to quantify the % metastatic area relative to total lung (total lung = 100%). (G) Representative H&E stains of lung metastases (red arrows) were found. For full data set and analyses, see Supplementary File S3. * p < 0.05; Two-way ANOVA.