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Removal of NHR1-1 from Prs1. (a) PRPP synthetase activity in WT or prs1Δ strains transformed with the vector, Prs1 or prs1(ΔNHR1-1); (b) Rlm1 expression after incubation at 30 °C and 37 °C for the strains shown in (a); (c) Y2H analysis of the interactions between Prs1/Prs3 and prs1(ΔNHR1-1)/Prs3 in pGBT9 and pGAD vectors carrying the DNA binding or activation domains of the S. cerevisiae Gal4 protein. p-values: * = p ≤ 0.05; ** = p ≤ 0.05, ns = not significant.
