Figure 6.

Contribution of the prs5(479) carrying phosphosite mutations, S₃₆₄A S₃₆₇A S₃₆₉A, in NHR5-2 of Prs5: (a) Rlm1 expression at the indicated temperatures for prs5Δ transformed with vector, Prs5 or prs5(479); (b) phosphorylation of Slt2 for the same strains as indicated in (a). The cultures were processed for Western blotting, and the membranes challenged with the indicated antibodies shown to the right of the figure in the presence or absence of heat shock. Actin is the loading control. The signals were detected by chemiluminescence. (c) Interaction of the yeast Gsk3 kinase, Rim11, with Prs5 and prs5(479). Positive control: Prs4/Prs5; (d) Fks2 expression for the same strains under the conditions described in (a). * p < 0.05, ** p < 0.01 (Tukey’s HSD test).