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. 2022 May 27;4(2):277–286. doi: 10.3390/clockssleep4020024

Figure 1.

Figure 1

Screening of 5-HT2c receptor antagonists from modified Yukmijihwang-tang using protein chip-based 5-HT2cR assay. For screening effective antagonists to the 5-HT receptor, candidate drugs, including YmP, were reacted to a Protein Chip bound to 5-HT2c receptors, and then Cy5-tryptamine was added. In the case of YmP, the fluorescence signal was decreased, and YmP was an effective antagonist. Red color represents fluorescence intensity to Cy5-labeled tryptamine alone bound to 5HT2c receptors, and yellow color represents competitively drug-induced inhibitory binding activity. Concentration of 5-HT2cR is 50 μg/mL and concentration of Cy5-labeled Tryptamine is 500 μM. Yukmijihwang-tang (Ym); modified Yukmijihwang-tang (YmP), various modified forms of Yukmijihwang-tang (Y1-5).