Figure 6.

Stimulation of THP-1 with nanovesicles from Lactobacillus johnsonii increases IL-10 expression in βlox5 via STAT3 activation. Co-culture of βlox5 and THP-1 cells were performed as described in the Materials and methods sections. (A–D) : blue bars represent the vehicle control group, whereas orange bar represents the NV10 treatment group. The mRNA levels of CYP1A1 (A), CYP1B1 (B), AHRR (C) and IL10 (D) were evaluated on βlox5 cells after co-cultured with THP-1 cells via qRT-PCR. Gene expression is shown relative to the vehicle control. (E) Western blot showing the total STAT3 (T-STAT3) and phosphorylation at S727 (S-STAT3) and Y705 (Y-STAT3). Three biological replicates are shown for each treatment as indicated. (F–H) Quantification of band intensity was performed with ImageJ and expressed as (F) T-STAT3/ACTIN, (G) S-STAT3/T-STAT3 and (H) Y-STAT3/STAT3 ratios. Blue bars represent the protein levels in βlox5 in the absence of THP-1, while the orange bars represent the protein levels in Blox5 after being co-cultured with THP-1 cells. CTRL denotes vehicle control group; NV10 denotes nanovesicles from L. johnsonii at 10¹⁰ particles/ml. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01.