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. 2022 Jun 16;11(12):1936. doi: 10.3390/cells11121936

Figure 6.

Figure 6

EGFR and U46619 synergistically regulate two cardiovascular relevant genes, Spp1 and Klf15. (a) FPM for Spp1 and Klf15 genes. The dotted line corresponds to the 5 FPM threshold employed to filter out lowly expressed genes (* p < 0.05—t-test). (b) Relative mRNA expression of Spp1 and Klf15 measured by qPCR. Outliers were removed, and the control group was used as a reference for each independent replicate. (n = 8–9, * p < 0.05—Wilcoxon test). (c) The amount of secreted OPN after 48 h incubation was measured by ELISA and normalized to the total amount of protein isolated from the cells cultivated with the measured media. The U46619-treated group served as a reference as the values for the control groups were close to the background ones (n = 4, * p < 0.05—Wilcoxon test).