TABLE 1.
Summary of the archaeal 16S rRNA sequences identified from the growth chamber deployment at Snake Pit, Mid-Atlantic Ridge
| Group | Type sequence(s)a | Other representative(s)b | % of clonesc | Closest relative | % Sequence similarity |
|---|---|---|---|---|---|
| Thermococcales | 71 | ||||
| VC2.1Arc1, -16 | VC2.1Arc5, -35 | Thermococcus spp. | 96.1–97.5 | ||
| VC2.1Arc7 | Thermococcus litoralis | 88.9 | |||
| Archaeoglobales | 22 | ||||
| VC2.1Arc4, -8 | VC2.1Arc2 | Archaeoglobus veneficus | 96.2–98.6 | ||
| VC2.1Arc36 | Archaeoglobus veneficus | 95.1 | |||
| Thermoplasmales | VC2.1Arc13 | 2 | pSSMCA108 | 97.1 | |
| Marine benthic group D (Euryarchaeota) | VC2.1Arc6 | 2 | pMC2A33 | 78.3 | |
| Marine benthic group B (Crenarchaeota) | VC2.1Arc31 | 2 | pMC2A36 | 86.3 |
a
The type 16S rRNA sequence was used in the maximum-likelihood and bootstrap analyses.
b
Additional closely related full-length sequences that were submitted to GenBank.
c
Percentage of clones that are closely related based on RFLP analysis and sequence analysis. This is not a percentage estimate of the relative abundance of these phylotypes in the sample.