TABLE 3.
Summary of the bacterial sequences identified from the growth chamber deployment at Snake Pit, Mid-Atlantic Ridge
| Group | Type sequence(s)a | Other representative(s)b | % of clonesc | Closest relative | % Sequence similarity |
|---|---|---|---|---|---|
| Aquificales | VC2.1Bac27 | VC2.1Bac10, -11, -28, -33, -39 | 18 | pBB | 92.3–92.6 |
| Desulfurobacterium | VC2.1Bac3, -24, -48 | VC2.1Bac2, -5, -13, -23, -34 | 25 | Desulfurobacterium thermolithotrophum | 93.4–96.0 |
| ɛ-Proteobacteria | VC2.1Bac32 | 3 | PVB_12 | 88.4 | |
| VC2.1Bac1, -31 | VC2.1Bac4 | 9 | SB17 | 90.7–92.6 | |
| VC2.1Bac43, -7, -17, -30 | VC2.1Bac8, -9, -12, -19, -20 | 28 | Unresolved | ||
| ɛ-Proteobacteria | VC2.1Bac29 | 3 | Leptothrix discophora | 96.4 | |
| Flexibacter-Cytophaga-Bacteroides | VC2.1Bac22 | 3 | SB5 | 89.7 | |
| Unresolved | VC2.1Bac16 | 3 | BD2-14 | 79.4 | |
| VC2.1Bac47 | 3 | Thermodesulforhabdus norvegicus | 82.8 | ||
| VC2.1Bac35 | 3 | Nitrococcus mobilis | 83.8 |
a
The type 16S rRNA sequence was used in the maximum-likelihood and bootstrap analyses.
b
Additional closely related full-length sequences that were submitted to GenBank.
c
Percentage of clones that are closely related based on RFLP analysis and sequence analysis. This is not a percentage estimate of the relative abundance of these phylotypes in the sample.