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. 2022 Jun 15;13(6):1067. doi: 10.3390/genes13061067

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Localization of bonafide TvACT and TvTUB during T. vaginalis karyokinesis and cytokinesis. Indirect immunofluorescence assay using anti-TvACT1r ((a3–f3); in green) and anti-TUB ((a4–f4); in red) homologous and heterologous antibodies, respectively, and DAPI for nuclear staining ((a2–f2); in blue). Nomarsky images (a1–f1). (a1–a5) Parasite in interphase; (b1–b5,c1–c5) parasite in karyokinesis; (d1–d5, e1–e5,f1–f5) parasite in cytokinesis; (a5–f5) signal merging of TvACT and TvTUB during mitosis. Dotted lines indicate magnified views of parasites in cytokinesis. A: axostyle; S: mitotic spindle dubbed paradesmose; white arrows: other tubulin-containing structures; white arrowheads: actin fibers; (*): membrane strangulation during cytokinesis. NTS: nanotube-like structure. Scale bar: 10 µm.