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Schematic representation of the process used to generate ACE-1 inhibitory permeates from L. digitata. Briefly, milled and dried L. digitata was heat deactivated and hydrolysed with two enzymes—Viscozyme® and Alcalase®. Hydrolysates were filtered to generate permeate and retentate fractions using a Millipore Lab scale TFF system and a 3 kDa cellulose filter. Fractions were subsequently tested for their ability to inhibit the ACE-1 enzyme using a spectrophotometric method.
