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. 2022 Jun 17;13(6):1082. doi: 10.3390/genes13061082

Figure 5.

Figure 5

The effect of TUBB4B-KO on mouse spermatogonia cell cycle. (A): Cell cycle detection of control mouse spermatogonia by flow cytometry; (B): Cell cycle detection of TUBB4B-KO mouse spermatogonia by flow cytometry; (C): Cell cycle analysis of control and TUBB4B-KO mouse spermatogonia; (D): The cell cycle gene expression between control and TUBB4B-KO mouse spermatogonia; (E): immunofluorescence to detect the expression of β-Tubulin protein in control mouse spermatogonia. The magnification was 200×, while the scale bar was 100 μm; (F): immunofluorescence to detect the expression of β-Tubulin protein in TUBB4B-KO mouse spermatogonia. The magnification and scale bar were the same as in €, and the green rectangles indicate the region of (G); (G): The zoomed-in (F) part. The scale bar was 20 μm. Furthermore, the G1 means the G0/G1 phase of the cell cycle, the G2 means the G2/M phase of the cell cycle. ‘*’ means 0.01 ≤ p-value < 0.05, ‘**’ means 0.001 ≤ p-value < 0.01, and ‘***’ means p-value < 0.001.