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. 2022 Jun 15;13(6):1070. doi: 10.3390/genes13061070

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenic relationship among Mediterranean and American L. infantum isolates (A,B). The evolutionary history was inferred using the maximum likelihood method and the Tamura–Nei model [43]. The tree with the highest log likelihood (−21,148.45) is shown. The tree is drawn to scale, with branch lengths representing the number of substitutions per site. This analysis involved 63 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. There was a total of 16,285 positions in the final dataset. Brazilian isolates; Spanish isolates; Tunisian isolates; Turkish isolates; Cypriot isolates; L. donovani (outgroup). All the evolutionary analyses were conducted in MEGA 11 [45].

Inline graphic — Phylogenic relationship among Mediterranean and American L. infantum isolates (A,B). The evolutionary history was inferred using the maximum likelihood method and the Tamura–Nei model [43]. The tree with the highest log likelihood (−21,148.45) is shown. The tree is drawn to scale, with branch lengths representing the number of substitutions per site. This analysis involved 63 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. There was a total of 16,285 positions in the final dataset. Brazilian isolates; Spanish isolates; Tunisian isolates; Turkish isolates; Cypriot isolates; L. donovani (outgroup). All the evolutionary analyses were conducted in MEGA 11 [45].