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. 2022 Jun 10;18(6):e1010606. doi: 10.1371/journal.ppat.1010606

Fig 1. D66 is a small molecule that prevents S. Typhimurium replication and/or survival in macrophages.

Fig 1

A). Structure of D66. B) RAW264.7 macrophage-like cells were infected with S. Typhimurium harboring a chromosomal sifB::gfp reporter, treated with DMSO or D66 at two hours after infection, and monitored for bacterial accumulation (GFP+ Macrophage Area) over 16 hours using the SAFIRE assay. The IC50 value is indicated. Mean and SD of biological duplicates with technical duplicates across 9 dilutions of D66. C, D) CFU assays were performed with RAW264.7 or HeLa cells infected with S. Typhimurium SL1344 or 14028 for two hours and treated for 16 hours with DMSO or 1.5-fold dilutions of D66, prior to lysis and plating for CFU. Symbols on the Y-axes are the CFU value from DMSO-treated samples. The IC50 values are indicated. Mean and SEMs of biological triplicates with technical duplicates across 8 dilutions of D66.