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. 2022 Jun 10;18(6):e1010606. doi: 10.1371/journal.ppat.1010606

Fig 2. D66 inhibits bacterial growth under conditions that damage the cell envelope and does not permeabilize the outer membrane.

Fig 2

A, B) Dose response curves monitoring S. Typhimurium (SL1344) or E. coli (K12) growth from an OD600 of 0.01, normalized to growth in 2% DMSO under the indicated condition. Mean and SEM of at least three biological replicates performed with technical triplicates; curve fit: sigmoidal, 4PL. C—E) Checkerboard assays of S. Typhimurium SL1344 growth from an OD600 of 0.01 in LB for 18 hours with novobiocin (up to 100 μg/mL) and (C) PMB (up to 48 μM), (D) JD1 (up to 150 μM), or (E) D66 (up to 150 μM). Growth was normalized to growth in 2% DMSO, with the darkest blue representing 100% growth and white representing 0%.