Fig 4.

CMV vaccine induces polyfunctional CMV-specific CD8⁺ and CD4⁺ T cells: In vitro expanded CMV-specific T cells from HLA expressing immunized mice were assessed for polyfunctional profile using multiparametric intracellular cytokine assay. These T cells were assessed for the expression of IFNγ, TNF and/or IL-2 simultaneously by flow cytometry in combination with SPICE software. Pie charts represent the proportion of CMV-specific CD8⁺ and CD4⁺ T cells expressing various combinations of IFNγ, TNF and/or IL2 cytokines after ex vivo and in vitro expansion. Arc indicates the proportion of CMV-specific CD8⁺ and CD4⁺ T cells expressing individual cytokines (IFNγ, TNF or IL-2).