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. 2022 Jun 10;18(6):e1010089. doi: 10.1371/journal.ppat.1010089

Fig 4. Cells lacking Tor1 N-terminal HEAT repeats were defective in oxygen consumption and hypersensitive to electron transport chain complex inhibitors.

Fig 4

A. Percentage of oxygen saturation of the medium in strains with distinct TOR1 alleles grown in YPD. Error bars show SD of 3 biological replicates. (TOR1/TOR1, JKC1713; tor1/TOR1, JKC1347; tetO-TOR1-Del381, JKC1441; tetO-TOR1, JKC1549). B. Cells of indicated genotypes were pre-grown in YPD and inoculated in YNB with 2% glucose without inositol, containing Vehicle (DMSO), Piericidin, Thenoyltrifluoroacetone (TTFA), Antimycin A or potassium cyanide (KCN) at the indicated concentrations. Error bars show SD of 3 technical replicates. (TOR1/TOR1, JKC1713; tor1/TOR1, JKC1347; tetO-TOR1-Del381, JKC1441; tetO-TOR1, JKC1549). The upper panel shows the actual growth curves and the lower panel shows the corresponding area under the curve (AUC) for each condition. ns is p = 0.080 (Piericidin); ** is p = 0.0026 (TTFA); * is p = 0.040 (Antimycin A) and p = 0.016 (KCN); error bars show SD of 3 technical replicates. Representative of 3 biological replicates.