Skip to main content
. 2022 Jun 10;18(6):e1010089. doi: 10.1371/journal.ppat.1010089

Fig 8. Plasma membrane stress responses did not significantly depend on Tor1 N-terminal HEAT repeats.

Fig 8

A. Dilutions of cells of indicated genotypes were spotted on YPD medium without or with doxycycline (300 Doxy) and plasma membrane stress was induced with 0.005% SDS. (TOR1/TOR1, JKC1713; tor1/TOR1, JKC1345, JKC1346, JKC1347; tetO-TOR1-Del381, JKC1442, JKC1445, JKC1441; tetO-TOR1, JKC1543, JKC1546, JKC1549). B. Cells of indicated genotypes were grown in YPD medium containing Vehicle (Veh, H2O) or 0.05% SDS, without or with 300 ng/ml doxycycline. Upper panel shows the actual growth curves and lower panel shows the corresponding area under the curve (AUC) for each strain. ** is p = 0.0076; * is p = 0.029 (Veh, 0 Doxy) and p = 0.0129 (0.05% SDS, 300 Doxy); ns is p = 0.2, error bars show SD of 2 biological replicates (TOR1/TOR1, JKC1713; tor1/TOR1, JKC1347; tetO-TOR1-Del381, JKC1441; tetO-TOR1, JKC1549). C. Cells of indicated genotypes were pre-grown in YPD medium with 5 ng/ml doxycycline for 3.5 h, then diluted into fresh YPD medium containing either Vehicle (Veh, H2O) or 0.01% SDS and incubated for the indicated times. Total protein extract was probed with antibody to phosphorylated Rps6 (P-S6) and tubulin (Tub) as loading control. Dens: signal intensity ratio of P-S6 to Tub; strains as in panel B. D. Cells were grown as in B but treated with 1 μg/ml Fluconazole (Fluc) or 0.2 μg/ml Amphotericin B (AmB).