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. 2022 Jun 16;23(12):6737. doi: 10.3390/ijms23126737

Figure 1.

Figure 1

In vitro functionality of EST79232 and EST79376 at Sig-1R: Sig-1R and BiP interaction assay. (A) Chemical structure of the two new compounds synthesized, EST79232 and EST79376 from the same chemical series and PRE-084. (B) Concentration-response curves of EST79232 and EST79376 on the Sig-1R/BiP interaction. (C,D) Agonist effect of 10 µM of PRE-084, and the antagonist effect of 10 µM of NE100. NE100 was able to revert the effect of PRE-084. EST79232 at 30 μM was able to reduce the association between Sig-1R and BiP, and this effect was reverted by NE100 (C). EST79376 at 30 µM had no effect on its own, but it was able to revert the effect of PRE-084 (D). Values are expressed as mean ± SEM of 6 determinations. *** p < 0.001 vs. control. One-way ANOVA, followed by Dunnett’s post-hoc multiple comparisons test.