Figure 2.

EST79232 and EST79376 ligands prevent MN death under chronic excitotoxicity. (A) Representative confocal images of spinal cord ventral horns labeled with SMI32 antibody at 28 DIV for control, THA and THA plus EST79232 or EST79376 treated at four concentrations (0.03, 0.3, 3, and 30 μM). Scale bar: 100 μm. (B,C) Bar graphs showing the number (mean ± SEM; n = 20–24 hemisections per treatment) of SMI-32-positive cells of each spinal cord hemislice. One-way ANOVA, followed by Bonferroni’s post-hoc test; **** p < 0.0001, *** p < 0.001; ** p < 0.01, * p < 0.05 vs. THA condition.