Figure 8.

Auranofin-treated cells have a dose-dependent decrease in SARS-CoV-2 RNA: Auranofin (0.1–10 μM) was applied to SARSCOV-2 infected Huh7 cells in repeated dilutions. RT-PCR was used to quantify viral RNA in cell pellets and culture supernatants using primers and probes targeting the SARS-CoV-2 N1. The data were graphed using a non-linear regression model (GraphPad software). Auranofin reduced virus replication in infected cells with an EC50 of about 1.4 μM. The 50% cytotoxic concentration was around 5.7 μM. The data represent two separate tests that were carried out in duplicate, with permission from [134].