Figure 2.

Analysis of secretome in 10% FBS-supplemented media. (A) A schematic diagram of two independent searches using human and bovine databases to minimize the false-positive identification possibly derived from 10% FBS. (B) Criteria to determine the origin of shared peptide between human and bovine proteins. Each horizontal bar indicates the whole sequence of a protein. The color box inside the bar indicates the peptides identified in each database, and the green box indicates the shared peptide between human and bovine proteins. Cross marks in black indicate that the peptide is assumed not to be derived from this protein. In the final decision, only proteins with two or more peptides are counted. (i) it was determined that only bovine protein was identified, (ii) determined that only human protein was identified, (iii) both bovine and human proteins were determined to be identified. (C) Number of identified proteins after enrichment using only the human database. (D) Number of identified proteins after enrichment using both the human and bovine databases and applying the criteria.