Figure 3.

Enrichment of ANL-labeled proteins in the secretome. (A) Optimization of the ratio between the conditioned media volume and the alkyne-containing bead volume. In our strategy, 15 mL of the conditioned medium is the optimal point in terms of the depth of the secretome profile. (B) Reproducibility of our enrichment strategy: 79.2% (or 70.3%) of proteins (or peptides) were identified in at least two out of three replicates. (C) The secretory pathway analysis of the identified proteins in secretome after enrichment using SignalP, SecretomeP, and TMHMM. (D) Gene ontology cellular component analysis of the identified proteins in secretome after enrichment.