TABLE 4.
Final cell density, pH, concentrations of CH4 and O2, and amounts of N2O, NO, NO2−, and NO3− per bottle in cultures of strain T20 incubated for 48 h in NMS or AMS medium in the presence of CH4 and 0 or 20% O2a
| Medium | CH4 (%) | O2 (%) | OD660 | pH | CH4 (%) | O2 (%) | N2O (pmol) | NO (pmol) | NO2− (pmol) | NO3− (μmol) |
|---|---|---|---|---|---|---|---|---|---|---|
| NMS | 20 | 0 | 0.17 | 6.6 | 20.4 | 0 | 3.5 | 4,066 | 720 | 102 |
| 20 | 0.69 | 6.4 | 6.0 | 0.64 | 5.0 | 115 | 1,293 | 78 | ||
| AMS | 1 | 0 | 0.15 | 6.5 | 0.99 | 0 | 0 | 26 | 9 | 0.10 |
| 20 | 0.15 | 6.1 | 0.37 | 19.5 | 0.14 | 35 | 26 | 0.18 | ||
| AMS | 20 | 0 | 0.20 | 6.3 | 20.0 | 0 | 0.19 | 29 | 0 | 0.10 |
| 20 | 0.31 | 4.6 | 16.1 | 15.1 | 0 | 32 | 0 | 0.10 |
a
All values are the averages for duplicate cultures. Under the conditions of this experiment, 4,066 pmol of NO is equivalent to a specific activity of 10−18 mol of NO cell−1 day−1. Experimental details are as for Table 1 except that initial OD660 values were 0.16 to 0.19.