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. 2022 Jun 17;23(12):6766. doi: 10.3390/ijms23126766

Figure 7.

Figure 7

Effect of cannabinoid agonists on stromal cell gene expression of fibroblast-related gene changes induced by A549-cell-conditioned medium (A549CM). The culture medium was conditioned with A549 cells cultured in the presence or absence of 5 ng/mL TGFβ, treated or not with an equimolar mixture of 10 µM THC + CBD. Then, NFs (A,C,E) or CAFs (B,D,F) were cultured with this conditioned medium for 72 h. The relative gene expression of COL1A1, VIM and FSP1 was studied by real-time RT-PCR using the GAPDH as housekeeping. Average fold change ± SD of 3 different experiments is represented. A triplicate was analyzed in each of the experiments. * p < 0.05 compared to the control group (white bars). # p < 0.05 compared to the NFs or CAFs cultured with A549CM in the absence of TGFβ or cannabinoid agonists (black bars). + p < 0.05 compared to the NFs or CAFs cultured with A549CM + TGFβ in the absence of cannabinoid agonists (gridded bars).