Figure 1.

Role of DMT1 in bone-marrow-derived macrophages (BMDMs): (a) Uptake (n = 4) and release assay (n = 2): BMDMs were stimulated with radioactive ⁵⁹Fe-citrate, and uptake/release was measured with a gamma-counter (counts per minute—cpm) and normalized to the protein concentration (µg). ⁵⁹Fe release was normalized to uptake data; (b) Relative mRNA expression (n = 5) of genes involved in iron transport: Ribosomal Protein L4 (Rpl4) was used as a reference gene (averages ± SEM); (c) BMDMs (n = 2) were stimulated with 50 µM FerricChloride (FeCl) or Deferoxamine (DFO) overnight. Protein levels of transferrin receptor 1 (TFR1), ferroportin (FPN1), ferritin (FRT), Poly(rC)-binding protein 2 (PCBP2), Nuclear Receptor Coactivator 4 (NCOA4) and βActin(ACTB); (d) Densitometric quantification of Western blot results; see Figure S5 for a schematic overview of the discussed proteins. Data were compared by a two-tailed unpaired t-test (two groups) or analysis of variance (ANOVA) using the Bonferroni correction (more than two groups); n = mice per group; Data are expressed as box plots showing whiskers with minimum to maximum. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, no significance of differences; DMT1: DMT1 fl/fl^LysMCre(+), WT: wildtype.