Figure 5.

Macrophage DMT1 protects against Salmonella infection in vivo (a) Mice were subjected to a lethal dose of Salmonella by injecting 500 colony-forming units (CFU) intraperitoneally (n = 15). Survival data between control and mutant mice were compared using Cox regression and the Kaplan–Meier method using Wilcoxon’s test. (b) CFU normalized to the weight of spleen or liver from mice infected with Salmonella after 72 h (n = 7) (c) Western Blot for selected genes from spleens 72 h after infections (n = 7), (d) Densitometric quantification of the Western Blot, (e) Tissue iron of the spleen normalized to protein and wildtype control (n = 7). Duplicates or triplicates from at least two independent experiments were compared by a two-tailed unpaired t-test (two groups) or analysis of variance (ANOVA) using a Bonferroni correction (more than two groups); n = mice per group. Error bars express the SEM. Box plots display whiskers with the. minimum to maximum. Statistical significance * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance of differences; DMT1: DMT1 fl/fl^LysMCre(+), WT: wildtype.