Mutation in Lox alters vascular mechanics. (A,B), SBP (A) and PP (B) are significantly higher in 3-month C57 Lox+/C285F mice (Mann–Whitney). (C), Aortic diameter is significantly smaller at pressures above 100 mmHg (C, two-way ANOVA p < 0.0001 for genotype and pressure, multiple comparison testing by Sidak) but dilates with age ((D), *—3 vs. 6 months, ^—3 vs. 12 months, and #—6 vs. 12 months; two-way ANOVA, Sidak). (E), Difference of average diameters over 3–6-, 6–12-, and 3–12-month intervals show a biphasic trajectory with the average C57 Lox+/C285F aorta dilating faster than C57 Lox+/+ early (Δ3–6 months) and leveling off later ((Δ6–12 months), for larger change in diameter over the Δ3–12-month period. For all tests *, # p < 0.05, **, ## p < 0.01, ### p < 0.001, and ****, ^^^^, #### p < 0.0001.