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. 2022 Jun 17;23(12):6749. doi: 10.3390/ijms23126749

Figure 7.

Figure 7

Mutation of Lox at C285F did not cause reduction in gene expression and Lox protein secretion as well as enzyme activity. (A), Lox and LoxL family members had similar mRNA expression in both Lox+/C285F and Lox+/+ mice as measured by quantitative PCR (t-test). (B), Lox+/C285F aorta showed 46% reduction in Lox enzyme activity in Lox+/285F mice as measured by Amplex Red (t-test; n = 8). **** p < 0.0001. (C), Total lysate from frozen aortas at age E19, P15, and P90 demonstrated higher quantities of 50 kDa proLox in LoxC285F/C285F as compared to Lox+/+ at age E19 only. However, a decreased amount of 30 kDa mature Lox in LoxC285F/C285F and/or Lox+/C285F was found in all three age groups. Graphs show the quantification of proLox and mature Lox at age (D), E19, (E), P15 and (F), P90 across all samples using total protein as normalization ((D) one-way ANOVA (p < 0.01 in both comparisons), Dunnet comparisons shown in figure; (E,F) Paired t-test). * p < 0.05; ** p < 0.01. (G) In conditioned medium collected from MEF isolated from Lox wild-type and mutant mice (n = 3), comparable amount of secreted mature Lox protein was detected. (Right panel, one-way ANOVA, Dunn).