Figure 7.

PERK deficiency reduces stiffness-dependent cell motility and proliferation. GG16-WT and PERK-KO spheroids were seeded on three hydrogels with increasing stiffness, as indicated (A). Cell migration was monitored during 48 h using brightfield microscopy. The surface area of the expanded spheroids was calculated and normalized to the surface area at 0 h (B,C). (D) Cell proliferation of GG16-WT and PERK-KO cells was determined after 6 days of growth on indicated stiff matrixes with Cell Tracker™ Green CMFDA Dye. Cell numbers were normalized to the 24 h point. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.