Experimental design: The control (CON) comprised a 20-min period of sitting on a standard chair, continuous exercise (CE) was performed as a 20-min bout of cycling on the cycle ergometer at lactate threshold (LT) intensity, and intermittent exercise (IE) was performed as 20 sets of a one-min bout of cycle ergometer exercise at LT intensity with a 30-s rest between every two sets. HR: heart rate, RPE: Borg rating of perceived exertion. HR measurement and blood sampling were performed 10 min before the onset of cycling (Rest), at the 25%, 50%, and 100% time points of exercise (E25%, E50%, and E100%), and at 10 min after running (A10min), while RPE was at E25%, E50%, and E100%. The closed arrow () and open arrow () indicate the timing of HR measurement and blood sampling and the timing of RPE measurement, respectively.