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Reactivity of SSI-HYBs to Ala-substituted peptides analysed by modified enzyme-linked immunosorbent assay. The control peptide CREACLQGWTEA was used for generation of substituted peptides where Cys1 and Cys5 were substituted with Ala, and a single peptide where both Cys residues were substituted with Ala. Absorbances were corrected for background reactivity by subtracting reactivity from non-coated wells.
