Table 2.
SSI-HYB reactivity to Ala-substituted peptides. Amino acids in brackets represent the amino acid substituted by Ala. N represent the number of substitutions that were not tolerated in relation to obtaining antibody binding. The peptide CREAALQGWTEA was used for generation of substituted peptides. ++ represents reactivity similar to the control, + represents medium reactivity, − represents no reactivity.
| [R]2 | [E]3 | [L]6 | [Q]7 | [G]8 | [W]9 | [T]10 | [E]11 | N | |
|---|---|---|---|---|---|---|---|---|---|
| SSI-HYB 385-01 | + | + | + | ++ | ++ | − | − | − | 3 |
| SSI-HYB 385-02 | + | − | − | + | − | − | − | ++ | 5 |
| SSI-HYB 385-03 | ++ | ++ | − | + | − | − | − | ++ | 4 |
| SSI-HYB 385-04 | ++ | ++ | − | + | − | − | − | ++ | 4 |
| SSI-HYB 385-06 | ++ | ++ | ++ | ++ | ++ | − | ++ | ++ | 1 |
| SSI-HYB 385-07 | ++ | ++ | − | ++ | ++ | − | ++ | − | 3 |
| SSI-HYB 385-08 | + | ++ | − | ++ | ++ | − | ++ | − | 3 |
| SSI-HYB 385-09 | ++ | ++ | − | ++ | ++ | − | ++ | − | 3 |