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. 2022 Jun 17;23(12):6790. doi: 10.3390/ijms23126790

Figure 4.

Figure 4

Palmitate and SIRT2 silencing promote lipid accumulation in HepG2 cells, while SIRT2 overexpression attenuates palmitate-mediated lipid deposition. (A) HepG2 cells were incubated with BSA (vehicle) or palmitate (0.5 mM) for 24 h. Representative images of ORO-stained HepG2 cells and quantification of ORO-stained lipid droplets by spectrophotometric analysis after isopropanol extraction. (B) Representative Western blots of SIRT2. SIRT2 levels were normalized to the levels of β-tubulin in the same lane. (C,D) Images of ORO-stained HepG2 cells infected with lentiviruses expressing either a short hairpin for control (shCTRL) and SIRT2 (shSIRT2) to silence SIRT2, as well as a vector with GFP sequence (GFP) and SIRT2 sequence (SIRT2) for the overexpression. These cells were incubated with either BSA or palmitate for 24 h and spectrophotometric analysis of stained lipids. (scale bar = 50 μm). Results are presented as mean ± SEM; n = 3 per group; * p < 0.05.