Figure 6.
Combination treatment of sorafenib and orlistat altered expressions of ferroptosis-related proteins in Huh7 and Huh7/SR cells. Protein expression changes were detected by Western blot in (A) Huh7 and (B) Huh7/SR cells after treatments, which caused similar changes in xCT, GPX4, and KEAP1 expressions but had different effects on NRF2 expression in both cell lines. Significantly, orlistat and combination treatment decreased KEAP1 levels in both cell lines; both treatments only elevated NRF2 level in Huh7 cells but had little effect on Huh7/SR cells. a, compared to Control; b, compared to Sorafenib; c, compared to Orlistat; 1 p < 0.05; 2 p < 0.01; 3 p < 0.001.