Figure 8.

Combination treatment of sorafenib and orlistat diminished the elevated 18F-FDG uptakes detected in Huh7/SR cells and altered the expressions of glycolysis-related proteins. (A) Equal numbers of cells were cultured in the medium containing ¹⁸F-FDG for an hour after receiving different treatments. Sorafenib treatment did not change the ¹⁸F-FDG uptakes in both cell lines. However, the ¹⁸F-FDG uptakes were suppressed by orlistat and combination treatment, and the reduction percentages were more prominent in Huh7/SR cells. (B) Huh7 cells had higher intracellular glycogen than Huh7/SR cells, indicating that excess glucose uptakes in Huh7/SR cells were utilized for energy production rather than storage. Changes in HK2, GAPDH, and PKM2 expressions caused by treatments in (C) Huh7 and (D) Huh7/SR cells were determined by Western blot. a, compared to Control; b, compared to Sorafenib; c, compared to Orlistat; ¹ p < 0.05; ** p < 0.01 between two groups.