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. 2022 Jun 16;23(12):6718. doi: 10.3390/ijms23126718

Figure 4.

Figure 4

Western blotting and lectin blotting analysis results. Binding of mAb 1A4-1 and mAb 18A11 to Muc21 transfectants expressing different glycoforms. (a–c) Western blotting (WB) and lectin blotting (LB) analysis with anti-FLAG mAb, mAb 18A11, mAb 1A4-1, Vicia villosa lectin (VVA), Arachis hypogaea lectin (PNA) and Wheat germ agglutinin (WGA). As indicated by + (treatment) and − (no treatment), cells were treated with sialidase or both sialidase and β-galactosidase. All lysates were immunoprecipitated (IP) with anti-FLAG-M2 affinity gel before SDS-PAGE. (a) WB and LB analysis with the lysate of CHO-K1-pcDNA3.1-N-FLAG-Muc21 cells (T) and the lysate of CHO-K1-pcDNA3.1 mock cells (M). (b) WB and LB analysis with the lysate of CHO-Lec2-pCAGGS-N-FLAG-Muc21 cells (T) and the lysate of CHO-Lec2-pCAGGS mock cells (M). (c) WB and LB analysis with the lysate of CHO-ldlD-pCAGGS-N-FLAG-Muc21 cells (T) and the lysate of CHO-ldlD-pCAGGS mock cells (M).